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Although the aquaculture industry contributes less than 0.2% to the Gross Domestic Product (GDP) of Malaysia, it has slowly become an important economic activity due to the high-value species productions for domestic and international markets. In addition, aquaculture can potentially be used as a sustainable solution for food security in the future. At present, the selection of aquaculture sites has not received much attention. Thus, this study aims to integrate a Geographic Information System and multi-criteria evaluation approach in identifying the potential sites for brackish aquaculture in the George Town Conurbation, Malaysia. ArcGIS 10.4 was used to perform site selection analysis together with the essential spatial data such as current land use, environmentally sensitive data, and soil quality that influence suitable sites selection for aquaculture. The selection was undertaken in ad hoc manners based on available land identified by aquaculture operators. The results indicated that the George Town Conurbation has a minimal potential site (0.37%) for aquaculture sites. This minimal number results from the expansion of built-up areas towards urban fringe areas; hence less land becomes available for aquaculture. A reasonable buffer zone should be designated as a boundary between urban development and aquaculture to avoid land-use conflict between these two activities.