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There was observed one-off effect of high nitrogen dose (1,000 kg per ha N) on the floristical composition of the Lolio-Cynosuretum R. Tx. 1937 association in the locality Chvojnica in the Strážovské vrchy during the four-year period (1997-2000). The high nitrogen dose supported only spread of four nitrophilous grass species (Dactylis glomerata L., Festuca pratensis HUDS., Festuca rubra L. and Deschampsia cespitosa L. P. B.) within the grassland. These species took up 89% of the share towards the end of experiment. Just three herbs species (Acetosella vulgaris FOURR., Cruciata glabra L. EHREND. and Veronica chamaedrys L.) continued in the coverage 1.5% with the interlude. The legumes (Trifolium pratense L., Trifolium repens L.) disappeared from the grassland in the first year of observation. The part of bare places from the first year of the experiment (46.7% - 1st cut) decreased to 8.75% (2nd cut) in the last year. The index of similarity oscillated from 0.47 to 0.57, which shows low degree of grassland similarity over the cuts, years, and also noticeable changes under the influence of high nitrogen dose. The landscape stability was affected negatively by the applied dose of nitrogen, and its value was very low S=0.51.
