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We examined the utility of nutrient criteria derived solely from total phosphorus (TP) concentrations in streams (regression models and percentile distributions) and evaluated their ecological relevance to diatom and algal biomass responses. We used a variety of statistics to characterize ecological responses and to develop concentration-based nutrient criteria (derived from ecological effects) for streams in Connecticut, USA, where urbanization is the primary cause of watershed alteration. Mean background TP concentration in the absence of anthropogenic land cover was predicted to be 0.017mg/l, which was similar to the 25th percentile of all study sites. Increased TP concentrations were significantly correlated with altered diatom community structure, decreased percent low P diatoms and diatoms sensitive to impervious cover, and increased percent high P diatoms, diatoms that increase with greater impervious cover, and chlorophyll a (P