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In support to the Remote Sensing Survey of the global Forest Resource Assessment 2010, the TREES-3 project has processed more than 12,000 Landsat TM and ETM+ data subsets systematically distributed over the tropics. The project aims at deriving area estimates of tropical forest cover change for the periods 1990–2000–2005. The paper presents the pre-processing steps applied in an operational and robust manner to this large amount of multi-date and multi-scene imagery: conversion to top-of-atmosphere reflectance, cloud and cloud shadow detection, haze correction and image radiometric normalization. The results show that the haze correction algorithm has improved the visual appearance of the image and significantly corrected the digital numbers for Landsat visible bands, especially the red band. The impact of the normalization procedures (forest normalization and relative normalization) was assessed on 210 image pairs: in all cases the correlation between the spectral values of the same land cover in both images was improved. The developed automatic pre-processing chain provided a consistent multi-temporal data set across the tropics that will constitute the basis for an automatic object-based supervised classification.