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In this paper we analyse the extent of fire-induced forest degradation in Mato Grosso State, Brazil. We utilise a sample based approach used in a previous pan-tropical deforestation survey to derive information on land cover and burned areas in the two major biomes of Mato Grosso: Amazon and Cerrado. Land cover and burned area are mapped for three years (2000–2005–2010) over 77 sample sites (10000ha each) distributed systematically throughout the state which covers 90.337 Mha. Our results indicate continuing forest degradation by fires in the state and potentially increasing fire susceptibility of the Amazon forests, regardless of the decrease in deforestation. 2010 witnessed the most extensive fire-induced forest degradation (~300000ha) in the forests of the Amazon biome among the study years, regardless of the fact that the fire season was less severe than in 2005. Deforestation in the Amazon biome in Mato Grosso dropped from 590000hayear-1 in the 2000–2005 period to 190000hayear-1 in the second half of the decade. The findings of this study advocate the inclusion of forest fire effects into carbon accounting initiatives.