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In a river, the flow directly affects the physical and chemical properties of its water, with further consequences for aquatic biota. Land use practices and vegetation cover play a significant role in the water cycle. The wide-spread perception of forest cover, in terms of hydrology is that forests may reduce water runoff: although in rare instances the contrary has been reported. Water runoff varies seasonally and depends on the forest tree species. By no means can it be considered constant over large expanses of area or for various rainfall patterns. In this paper, the results of a long-term hydrological survey conducted in two experimental microbasins (operated by the Institute of Hydrology SAS, IH SAS) with different land use practices are presented. The Rybárik microbasin (0.119 km2) is dominated by row crop production. The basin was 70% cultivated by the state farm and 30% by a private farm. The Lesný microbasin (0.086 km2) is covered by a deciduous hornbeam regrowth forest (Carpinus betulus). The analysis revealed that the difference in the runoff from the forest and the agricultural land increases with increasing precipitation; however, at some point (extreme precipitations with low probability) the runoff from these basins is nearly equal.