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Ecological Footprint Method is introduced to define the range of biological resource consumption account and fossil energy consumption account. According to the relevant data of land use and ecological environment in Huadu District, Guandzhou City, Guangdong Province, China from the year 1990 to 2005, ecological footprint analysis method is used to research on the demand and supply of land consumption function of Huadu District, as well as the difference variation between supply and demand. Result shows that per capita ecological footprint and total ecological footprint have increased sharply from 1990 to 2005. Biological resource consumption has an absolute advantage of proportion compared with fossil energy. Growth speed of fossil energy footprint is faster than that of biological resource footprint. However, structure of total ecological footprint has not changed significantly. Demand of land consumption function is far beyond the ecological carrying capacity of Huadu District. But land use intensity has not exceeded the ecological threshold produced by supporting consumption function of land use system, indicating that ecological environment system is still relatively safe in Huadu District.