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The urban agglomeration at the Yangtze River Delta is one of the six most developed and populated urban agglomerations in the world. In recent years, with accelerating urbanization, the land use has changed significantly. Excessive construction aggravates ecological fragility. In this context, this paper first investigates the evolutionary processes and layout of the ecological space in the Yangtze River Delta. The root causes of various problems are then analyzed. Finally, suggestions for further improvement in both detailed tasks and governance aspects are proposed. The applied methods included use of remote sensing (RS), geographic information system (GIS) and statistical analysis. Main results indicate that from 1990 to 2018, the ecological space of the Yangtze River Delta shrank, especially in the city of Shanghai. Ecological space is insufficient in the area, unevenly distributed and fragmented. The major root causes include rapid urbanization without overall planning, regional population surge and improper industrial structure. Suggestions for improvement include overall planning and integrated management, control of population size, active industry structure upgrade, land-use efficiency balanced with ecological indicators, and multiple integrated strategies encouraging application of new energy technologies.